期刊
MICROCHIMICA ACTA
卷 189, 期 4, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-022-05250-4
关键词
Dual immunoplatform; BAFF; APRIL; MoS2/MWCNTs; Amperometry; Serum; SLE; CRC
资金
- Spanish Ministerio de Ciencia, Innovacion y Universidades [PRE2019-087,596, RTI2018-096,135-B-I00]
- Spanish Ministerio de Ciencia e Innovacion [PID2019-103899RB-I00]
- TRANSNANOAVANSENS-CM Program from the Comunidad de Madrid [S2018/NMT-4349]
- AES-ISCIII program [PI17CIII/00045, PI20CIII/00019]
- Spanish Ministerio de Educacion, Cultura y Deporte
- Flanders Research Foundation (FWO) [1193818 N]
- CRUE-CSIC agreement
- Springer Nature
A dual immunosensor was developed for the simultaneous determination of two important immunity-related cytokines, BAFF and APRIL. The immunosensor showed high sensitivity and low detection limits, making it suitable for detecting these cytokines in cancer cell lysates and serum samples.
A dual immunosensor is reported for the simultaneous determination of two important immunity-related cytokines: BAFF (B cell activation factor) and APRIL (a proliferation-induced signal). Sandwich-type immunoassays with specific antibodies (cAbs) and a strategy for signal amplification based on labelling the detection antibodies (dAbs) with binary -MoS2/ MWCNTs nanostructures and using horseradish peroxidase (HRP) were implemented. Amperometric detection was carried out at screen-printed dual carbon electrodes (SPdCEs) through the hydroquinone HQ/H2O2 system. The developed dual immunosensor provided limit of detection (LOD) of 0.08 and 0.06 ng-mL(-1) for BAFF and APRIL, respectively, and proved to be useful for the determination of both cytokines in cancer cell lysates and serum samples from patients diagnosed with autoimmune diseases and cancer. The obtained results agreed with those found using ELISA methodologies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据