期刊
MICROCHEMICAL JOURNAL
卷 175, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.microc.2021.107171
关键词
Fluorescence; SERS; Gold nanoparticles; Dual-mode probe; Telomerase activity
资金
- National Natural Science Foundation of China [21974055, 21605062, 22171110, 22001099]
- Fund of Xuzhou Science and Technology Key R&D Program (Social Development) Project [KC20178]
A dual-mode functional nanosensor based on fluorescence and surface-enhanced Raman scattering (SERS) was proposed for monitoring telomerase activity. The sensor uses Cy5-functional gold nanoparticles (Au NPs) as the dual functional nanoprobe. Changes in the distance between the signaling molecule Cy5 and Au NPs result in changes in the SERS and fluorescence signals, allowing for telomerase activity monitoring.
The in situ assay of telomerase activity is important for cancer diagnosis. Herein, a dual-mode functional nanosensor based on fluorescence and surface-enhanced Raman scattering (SERS) was proposed for telomerase activity monitoring. The as-prepared biosensor features Cy5-functional Au nanoparticles (NPs) as the dual functional nanoprobe. The telomerase substrate primer extended under the action of telomerase to form the telomere repeats, and the hairpin DNA loop was opened during hybridization. The distance between the signaling molecule Cy5 and Au NPs changed, resulting in a decrease and a recovery of the SERS and fluorescence signal, respectively. The results showed that the fluorescence and SERS intensity both depended on the telomerase activity and could be used for sensing purposes. On this basis, in situ fluorescence combined with Raman imaging permitted real-time analysis of changes in the telomerase activity of human cells, and hence the approach could be applied for screening of anticancer drugs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据