Smartphone-based colorimetric method for determining sulfites in wine using a universal clamp sample holder and microfluidic cotton swab-based analytical device

A universal clamp sample holder was newly designed and fabricated for use with a microfluidic cotton swab-based analytical device (mu CSAD) in smartphone-based colorimetric determination of sulfites in wine. In the process, sulfite (SO32-) was converted to volatile SO2 by acidification, the SO2 reduced Fe(III) to Fe(II) which finally formed the complex of Fe(II)-phenanthroline with a red color. The latter two reactions occurred on a cotton swab head hanging over the sample solution in a vial. Then, the swab was placed in the sample holder that also contained a lens and a light-emitting diode, to be photographed by the built-in camera of the smartphone. A mobile software application was designed to generate the calibration equation and evaluate the amount of sulfites in the sample. The linear ranges were 0.30-2.25 as SO2 (R-2 = 0.9931) and 2.25-18.75 mg L-1 as SO2 (R-2 = 0.9956). The limit of detection was 100 mu g L-1. Compared to the Ripper method, the developed method showed no significant differences in measuring the sulfites in wine samples. This mu CSAD is simple, cost-effectiveness, and eco-friendly. Moreover, the designed universal clamp holder and the software application can be used with multiple brands and models of smartphones.

Automated summary

A universal clamp sample holder was designed and fabricated for smartphone-based colorimetric determination of sulfites in wine using a microfluidic cotton swab-based analytical device. The method involves converting sulfite to volatile SO2, which reacts with Fe(III) to form a red complex. The mu CSAD is cost-effective and eco-friendly, with a mobile software application for calibration and sulfite concentration evaluation.