期刊
MICROBIAL PATHOGENESIS
卷 166, 期 -, 页码 -出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2022.105539
关键词
Peptide vaccine; Enolase; Felis catus; Sporothrix brasiliensis; Sporotrichosis
资金
- 'Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior' (CAPES) [CAPES/PNPD-88887,474571/2020-00]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2015/09340-4, 2017/13228-0, 2017/26774-3]
- 'Conselho Nacional de Pesquisa e Desenvolvimento Tecnologico' (CNPq)
This study identified and validated six low-sequence-identity peptides from SsEno, which exhibit B-cell epitopes and react strongly with serum from infected mice and cats with sporotrichosis. Immunization with a peptide mixture induced high levels of protective antibodies in mice and elicited a cytokine profile associated with protection against sporotrichosis. These peptides could be potential safe antigens for developing vaccine strategies against feline sporotrichosis.
Sporotrichosis is a cosmopolitan mycosis caused by pathogenic species of Sporothrix genus, that in Brazil is often acquired by zoonotic transmission involved infected cats with S. brasiliensis. Previous studies showed that the Sporothrix spp. recombinant enolase (rSsEno), a multifunctional protein with immunogenic properties, could be a promising target for vaccination against sporotrichosis in cats. Nevertheless, the considerable sequence identity (62%) of SsEno with its feline counterpart is a great concern. Here, we report the identification in silico, chemical synthesis and biological validation of six peptides of SsEno with low sequence identity to its cat orthologue. All synthesized peptides exhibit B-cell epitopes on the molecular surface of SsEno and proved to be highly reactive with the serum of infected mice with S. brasiliensis and sera of cats with sporotrichosis. Interestingly, our study revealed that anti-peptide sera did not react with the recombinant enolase from Felis catus (cats, rFcEno), thus, may not trigger autoimmune response in these felines if used as a vaccine antigen. The immunization with peptide mixture (PeptMix) formulated with Freund adjuvant (FA), induced high levels of antigen-specific IgG, IgG1 and IgG2b antibodies that conferred protection upon passive transference in infected BALB/c mice with S. brasiliensis. We also observed, that the FA+PeptMix formulation induced a Th1/Th2/Th17 cytokine profile ex vivo, associated with protecting effect against the experimental sporotrichosis. Our results suggest that the six SsEno-derived peptides here evaluated, could be used as safe antigens for the development of vaccine strategies against feline sporotrichosis, whether prophylactic or therapeutic.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据