期刊
MARINE DRUGS
卷 20, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/md20050274
关键词
thermophilic; psychrostable; heavy metals; commercial esterases; brine pool; metagenomics
资金
- Proof Concept fund centennial grant from the American University in Cairo office of the Associate Provost for Research Innovation and Creativity
This study comprehensively evaluates the extreme properties of EstATII-TM lipase isolated from the Red Sea Atlantis II brine pool, showing its tolerance to high temperatures, stability to hot and cold conditions, and resistance to toxic heavy metals. EstATII-TM exhibits superior catalytic activity and resistance/enhancement to metal ions compared to commercial esterases.
Lipolytic enzymes catalyze the hydrolysis and synthesis of ester compounds. They are valuable in the pulp, food, and textile industries. This study aims to comprehensively evaluate the extreme properties of a hormone-sensitive lipase (EstATII-TM) isolated from the Red Sea Atlantis II brine pool. EstATII-TM was cloned, expressed, and its biochemical activities were assessed under different conditions. EstATII-TM catalytic properties and resistance to different metal ions were compared to commercial thermophilic esterases under different temperatures. Phylogenetically, EstATII-TM was assigned to the GDSAG motif subfamily of hormone-sensitive lipase. The optimal enzyme activity was evident at a temperature of 30 degrees C and pH 7-8. The enzyme retained 84.9% of its activity at 0.5 M NaCl. EstATII-TM maintained 93% to 97% activity at -40 and -20 degrees C, respectively. EstATII-TM activity was significantly enhanced, up to 10-fold, at temperatures ranging from 45 to 65 degrees C in the presence of 1 mM Cu2+, Cd2+, Ba2+, Mn2+, and Zn2+. EstATII-TM showed superior catalytic activity and resistance-to/enhancement-by metal ions compared to two commercial thermophilic esterases. The Red Sea Atlantis II brine EstATII-TM is characterized by tolerance to high temperatures, stability to hot and cold conditions, as well as toxic heavy metal contamination, making it an ideal candidate for industrial processes.
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