期刊
MARINE DRUGS
卷 20, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/md20050296
关键词
COVID-19; SARS-CoV-2; seaweed; abalone viscera; polysaccharide; antiviral activity
资金
- Ministry of Education from Republic of Korea [2017R1A6A1A03015876]
- Wando County
Crude polysaccharides extracted from seaweed and abalone viscera effectively inhibit the propagation of SARS-CoV-2 by interfering with viral entry.
Crude polysaccharides, extracted from two seaweed species (Hizikia fusiforme and Sargassum horneri) and Haliotis discus hannai (abalone) viscera, were evaluated for their inhibitory effect against SARS-CoV-2 propagation. Plaque titration revealed that these crude polysaccharides efficiently inhibited SARS-CoV-2 propagation with IC50 values ranging from 0.35 to 4.37 mu g/mL. The crude polysaccharide of H. fusiforme showed the strongest antiviral effect, with IC50 of 0.35 mu g/mL, followed by S. horneri and abalone viscera with IC50 of 0.56 and 4.37 mu g/mL, respectively. In addition, immunofluorescence assay, western blot, and quantitative RT-PCR analysis verified that these polysaccharides could inhibit SARS-CoV-2 replication. In Vero E6 cells, treatment with these crude polysaccharides before or after viral infection strongly inhibited the expression level of SARS-CoV-2 spikes, nucleocapsid proteins, and RNA copies of RNA-dependent RNA-polymerase and nucleocapsid. These results show that these crude marine polysaccharides effectively inhibit SARS-CoV-2 propagation by interference with viral entry.
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