期刊
MARINE DRUGS
卷 20, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/md20050308
关键词
Merluccius productus; Matrix metalloproteinase; type I procollagen; MAPK/AP-1; NF-kappa B; Nrf-2/HO-1; TGF-beta/Smad
资金
- Pacific Seafood
Pacific whiting skin gelatin hydrolysate (PWG) has anti-wrinkle effects by restoring collagen synthesis, inhibiting collagen degradation, reducing inflammation, and increasing antioxidant enzyme activity. The molecular mechanisms involve activation of the TGF-β/Smad pathway for collagen synthesis, inhibition of the MAPK/AP-1 pathway for collagen degradation, suppression of the NF-κB pathway for inflammation, and activation of the Nrf-2/HO-1 pathway for antioxidant defense.
Chronic exposure to ultraviolet (UV) light promotes the breakdown of collagen in the skin and disrupts the extracellular matrix (ECM) structure, leading to skin wrinkling. Pacific whiting (Merluccius productus) is a fish abundant on the Pacific coast. In the current study, we investigated the anti-wrinkle effect of hydrolysate from Pacific whiting skin gelatin (PWG) in UVB-irradiated human dermal fibroblasts and the molecular mechanisms involved. PWG effectively restored type 1 procollagen synthesis reduced by UVB-irradiation. Also, we found that PWG inhibited collagen degradation by inhibiting MMP1 expression. Furthermore, PWG decreased cytokines TNF-alpha, IL-6, and IL-1 beta associated with inflammatory responses and increased antioxidant enzymes, HO-1, SOD, GPx, CAT, and GSH content, a defense system against oxidative stress. In terms of molecular mechanisms, PWG increased collagen synthesis through activating the transforming growth factor beta (TGF-beta)/Smad pathway and decreased collagen degradation through inhibiting the mitogen-activated protein kinases/activator protein 1 (MAPK/AP-1) pathway. It also suppressed the inflammatory response through suppressing the nuclear factor-kappa B (NF-kappa B) pathway and increased antioxidant enzyme activity through activating the nuclear factor erythroid 2/heme oxygenase 1 (Nrf-2/HO-1) pathway. These multi-target mechanisms suggest that PWG may serve as an effective anti-photoaging material.
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