期刊
MARINE BIOTECHNOLOGY
卷 24, 期 1, 页码 226-242出版社
SPRINGER
DOI: 10.1007/s10126-022-10102-6
关键词
G protein-coupled receptor; Genome; Marine rotifer; Brachionus manjavacas
资金
- National Research Foundation [2020R1F1A1076854]
- Collaborative Genome Program of the Korea Institute of Marine Science and Technology Promotion - Ministry of Oceans and Fisheries [20180430]
- National Research Foundation of Korea [2020R1F1A1076854] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
In this study, the whole-genome sequence of the marine rotifer Brachionus manjavacas was assembled, and 310 GPCR genes were identified and classified. The results provide clues for studying GPCR-based signaling pathways in B. manjavacas and expand our understanding of the response of rotifers to various environmental signals.
The marine rotifer Brachionus manjavacas is widely used in ecological, ecotoxicological, and ecophysiological studies. The reference genome of B. manjavacas is a good starting point to uncover the potential molecular mechanisms of responses to various environmental stressors. In this study, we assembled the whole-genome sequence (114.1 Mb total, N50 = 6.36 Mb) of B. manjavacas, consisting of 61 contigs with 18,527 annotated genes. To elucidate the potential ligand-receptor signaling pathways in marine Brachionus rotifers in response to environmental signals, we identified 310 G protein-coupled receptor (GPCR) genes in the B. manjavacas genome after comparing them with three other species, including the minute rotifer Proales similis, Drosophila melanogaster, and humans (Homo sapiens). The 310 full-length GPCR genes were categorized into five distinct classes: A (262), B (26), C (7), F (2), and other (13). Most GPCR gene families showed sporadic evolutionary processes, but some classes were highly conserved between species as shown in the minute rotifer P. similis. Overall, these results provide potential clues for in silico analysis of GPCR-based signaling pathways in the marine rotifer B. manjavacas and will expand our knowledge of ligand-receptor signaling pathways in response to various environmental signals in rotifers.
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