期刊
MACROMOLECULAR BIOSCIENCE
卷 22, 期 7, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.202200005
关键词
calcium carbonate; drug release; hybrid particles; immobilization; protease activity; sorption
资金
- Staedtler Foundation [121041500039-8]
Local delivery of proteolytic enzymes is an alternative to antibiotic treatment, and this study proposes hybrid microspheres made of mucin and biodegradable crystals as carriers for chymotrypsin delivery. The encapsulated chymotrypsin shows preserved enzymatic activity and enhanced stability.
While the enteral delivery of proteolytic enzymes is widely established for combating many diseases as an alternative to antibiotic treatment, their local delivery only emerges as administration route enabling sustained release in a controlled manner on site. The latest requires the development of drug delivery systems suitable for encapsulation and preservation of enzymatic proteolytic activity. This study proposes hybrid microspheres made of mucin and biodegradable porous crystals of calcium carbonate (CC) as the carriers for chymotrypsin (CTR) delivery. CTR is impregnated into CC and hybrid CC/mucin (CCM) microspheres by means of sorption without any chemical modification. The loading of the CC with mucin enhances CTR retention on hybrid microspheres (adsorption capacity of approximate to 8.7 mg g(-1) vs 4.7 mg g(-1)), recharging crystal surface due to the presence of mucin and diminishing the average pore diameter of the crystals from 25 to 8 nm. Mucin also retards recrystallization of vaterite into nonporous calcite improving stability of CCM microspheres upon storage. Proteolytic activity of CTR is preserved in both CC and CCM microspheres, being pH dependent. Temperature-induced inactivation of CTR significantly diminishes by CTR encapsulation into CC and CCM microspheres. Altogether, these findings indicate promises of hybrid mucin-vaterite microspheres for mucosal application of proteases.
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