4.7 Review

From DNA break repair pathways to CRISPR/Cas-mediated gene knock-in methods

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Summary: The novel CRISPR/Cas9 based approach TKIT allows efficient and precise genomic knock-in of large DNA fragments, particularly challenging in non-dividing cells like neurons. By targeting non-coding regions, TKIT is resistant to INDEL mutations and has shown high efficiency, up to 42%, in labeling endogenous synaptic proteins. Utilizing in utero electroporation or viral injections in mouse models, TKIT can be used to visualize endogenous proteins and assess their mobility, highlighting its broad potential for precise genome editing in various model organisms.
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