期刊
LANGMUIR
卷 38, 期 18, 页码 5464-5471出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.langmuir.1c03456
关键词
-
资金
- KAKENHI [15H03541]
- Japan Society for the Promotion of Science (JSPS)
- Grants-in-Aid for Scientific Research [15H03541] Funding Source: KAKEN
This study investigates membrane protein reconstitution and fusion dynamics using artificial planar bilayer lipid membranes (BLMs) and baculovirus-budded virus (BV) systems. The results demonstrate that BV fusion with BLMs is enhanced at lower pH and can effectively reconstitute membrane proteins. The study reveals the promising potential of BV fusion for the development of biodevices to examine membrane protein activity.
Artificial planar bilayer lipid membranes (BLMs) are simple models of cellular systems under physically and chemically controlled conditions, and they have been used to investigate membrane protein activity. Baculovirus-budded virus (BV) systems can express recombinant membrane proteins. In this study, aiming for membrane protein reconstitution, we examined the fusion of BVs containing recombinant membrane proteins into artificial planar BLMs on a Si microwell substrate. BV fusion with the BLMs depended on the pH of the solution, and it was enhanced at lower pH. Based on fluorescence recovery after photobleaching (FRAP) measurement, the fusion state of BVs was evaluated, and full fusion at low pH was confirmed. The fluorescent labeling the membrane proteins was also observed in the freestanding part of the BLMs as well as in the supported part. These results demonstrate the effectiveness of BLMs as a platform to examine detailed fusion dynamics of BVs. Furthermore, this study revealed that the fusion of BVs is a promising method for reconstituting membrane proteins to artificial freestanding BLMs for the development of biodevices with which we can examine membrane protein activity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据