期刊
JOURNAL OF VIROLOGY
卷 96, 期 9, 页码 -出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/jvi.01957-21
关键词
African swine fever virus; A137R protein; IFN-beta; TBK1; autophagy-mediated lysosomal pathway
类别
资金
- National Natural Science Foundation of China [U20A2060, 32072855, 32072854, 32072866]
- Natural Science Foundation of Heilongjiang Province [YQ2020C025, JQ2020C002]
African swine fever (ASF) is a lethal viral disease of pigs caused by African swine fever virus (ASFV). A recent study found that deleting the A137R gene in ASFV attenuates its virulence and promotes the expression of type I interferon (IFN) in primary porcine alveolar macrophages. The A137R protein negatively regulates the cGAS-STING-mediated IFN-beta signaling pathway through the autophagy-mediated lysosomal degradation of TBK1. This study enhances the understanding of ASFV immunoevasion strategies and provides new insights for the development of live attenuated ASF vaccines.
African swine fever is a lethal hemorrhagic disease of pigs caused by African swine fever virus (ASFV), which greatly threatens the pig industry in many countries. Deletion of virulence-associated genes to develop live attenuated ASF vaccines is considered to be a promising strategy. A recent study has revealed that the A137R gene deletion results in ASFV attenuation, but the underlying mechanism remains unknown. To elucidate the mechanism of the A137R gene regulating ASFV virulence, an ASFV mutant with the A137R gene deleted (ASFV-Delta A137R) was generated based on the wild-type ASFV FILJ/2018 strain (ASFV-WT). Using transcriptome sequencing analysis, we found that ASFV-Delta A137R induced higher type I interferon (IFN) production in primary porcine alveolar macrophages (PAMs) than did ASFV-WT. Overexpression of the A137R protein (pA137R) inhibited the activation of IFN-beta or IFN-stimulated response element. Mechanistically, pA137R interacts with TANK-binding kinase 1 (TBK1) and promotes the autophagy-mediated lysosomal degradation of TBK1, which blocks the nuclear translocation of interferon regulator factor 3, leading to decreased type I IFN production. Taken together, our findings clarify that pA137R negatively regulates the cGAS-STING-mediated IFN-beta signaling pathway via the autophagy-mediated lysosomal degradation of TBK1, which highlights the involvement of pA137R regulating ASFV virulence. IMPORTANCE African swine fever (ASF) is a lethal viral disease of pigs caused by African swine fever virus (ASFV). No commercial vaccines and antiviral treatments are available for the prevention and control of the disease. Several virulence-associated genes of ASFV have been identified, but the underlying attenuation mechanisms are not clear. Compared with the virulent parental ASFV, the A137R gene-deleted ASFV mutant promoted the expression of type I interferon (IFN) in primary porcine alveolar macrophages. Further analysis indicated that the A137R protein negatively regulated the cGAS-STING-mediated IFN-beta signaling pathway through targeting TANK-binding kinase 1 (TBK1) for autophagy-mediated lysosomal degradation. This study not only facilitates the understanding of ASFV immunoevasion strategies, but also provides new clues to the development of live attenuated ASF vaccines.
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