期刊
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
卷 128, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jmbbm.2022.105102
关键词
Indentation; Raman spectroscopy; Endochondral ossification; Poroelastic; Physis
资金
- NIH [R21/R33 HD090696]
- University of Colorado Gates Grubstake Fund
- [RRID:SCR_019307]
Fractures in the growth plate can lead to the loss of bone lengthening function. Our understanding of growth plate fractures and mechanobiology is limited by the lack of information on the microscale spatial gradients in mechanical properties. In this study, we characterized the mechanical properties of the growth plate and found differences between different zones, which correlated with the content of extracellular matrix.
The growth plate is a cartilaginous tissue that functions to lengthen bones in children. When fractured, however, the growth plate can lose this critical function. Our understanding of growth plate fracture and mechanobiology is currently hindered by sparse information on the growth plate's microscale spatial gradients in mechanical properties. In this study, we performed microindentation across the proximal tibia growth plate of 9-week-old New Zealand White rabbits (n = 15) to characterize spatial variations in mechanical properties using linear elastic and nonlinear poroelastic material models. Mean indentation results for Hertz reduced modulus ranged from 380 to 690 kPa, with a peak in the upper hypertrophic zone and significant differences (p < 0.05) between neighboring zones. Using a subset of these animals (n = 7), we characterized zonal structure and extracellular matrix content of the growth plate through confocal fluorescent microscopy and Raman spectroscopy mapping. Comparison between mechanical properties and matrix content across the growth plate showed that proteoglycan content correlated with compressive modulus. This study is the first to measure poroelastic mechanical properties from microindentation across growth plate cartilage and to discern differing mechanical properties between the upper and lower hypertrophic zones. This latter finding may explain the location of typical growth plate fractures. The spatial variation in our reported mechanical properties emphasize the heterogeneous structure of the growth plate which is important to inform future regenerative implant design and mechanobiological models.
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