期刊
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
卷 33, 期 4, 页码 649-659出版社
AMER CHEMICAL SOC
DOI: 10.1021/jasms.1c00298
关键词
mass spectrometry imaging; microbiome; brain; neurotransmitters; metabolites
资金
- Biotechnology and Biological Sciences Research Council (BBSRC) -CASE studentship - AstraZeneca
- BBSRC [BB/K008005/1, BB/P003281/1]
- Wellcome Trust [097820/Z/11/B]
- Wellcome Trust [097820/Z/11/B] Funding Source: Wellcome Trust
- BBSRC [BB/K008005/1, BB/P003281/1] Funding Source: UKRI
Microbes have significant effects on the levels of neurotransmitters and their precursors in the brain. Antibiotic treatment does not cause significant changes in these metabolites in the brain.
Microbes exert influence across the microbiome-gut-brain axis through neurotransmitter production, induction of host immunomodulators, or the release or induction of other microbial or host molecules. Here, we used mass spectrometry imaging (MSI), a label-free imaging tool, to map molecular changes in the gut and brain in germ-free, antibiotic-treated and control mice. We determined spatial distribution and relative quantification of neurotransmitters and their precursors in response to the microbiome. Using untargeted MSI, we detected a significant change in the levels of four identified small molecules in the brains of germ-free animals compared to controls. However, antibiotic treatment induced no significant changes in these same metabolites in the brain after 1 week of treatment. This work exemplifies the utility of MSI as a tool for the study of known and discovery of novel, mediators of microbiome-gut-brain axis communication.
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