A novel electrochemical biosensor based on the electrospun nanofibrous nanocomposites of PCL-PPy-MWCNT towards determination of TNF-α biomarker

For TNF detection, a novel electrochemical biosensor based on the PCL-PPY-MWCNT electrospun nanofiber was developed in this study. TNF is a potent inflammatory adipokine that has been linked to a variety of pathological conditions, including heart disease. Although a normal heart does not produce TNF, a failing heart produces large quantities. Furthermore, there is a direct relationship between TNF levels and disease severity. As a result, low-level TNF alteration diagnosis is expected to be a prognosis factor for heart status. TNF antibody was immobilized onto the PCL-PPY-MWCNT electrospun scaffold electrode surface in this study, and TNF was quantified using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The biosensor detected TNF-alpha over a linear range of 1 to 1000 pg ml(-1) with a detection limit of 1 pg ml(-1), making it very promising for rapid analysis to detect heart attacks. Also, our biosensor showed a good response in several electrolytes, including probable interfering ions at biological concentrations. The electrochemical biosensor offers great promise for sensitive, rapid, and cost-effective analysis of biological samples.

Automated summary

A novel electrochemical biosensor based on the PCL-PPY-MWCNT electrospun nanofiber was developed for TNF detection, which showed great promise in sensitive and rapid analysis of heart disease prognosis factor.