Molecularly imprinted polymer on indium tin oxide substrate for bovine serum albumin determination

In this paper, a molecularly imprinted polymer (MIP) was electrodeposited onto the indium tin oxide (ITO) substrate for bovine serum albumin (BSA) determination. The films were prepared through the electrodeposition of BSA/polyaniline on ITO electrodes. Different characterization techniques analyzed MIP and non-imprinted (NIP) fabrication stages: Fourier-transform infrared spectroscopy, scanning electron microscopy (SEM), atomic force microscopy (AFM), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy. The results of the SEM and AFM revealed unique morphological characteristics of MIP films, with the formation of cavities after the template removal process, evidencing noticeable differences when compared to NIP films. In the electrochemical characterization were registered changes in the electric response of MIP films (increase current values with a subsequent decrease) during the BSA extraction-rebinding. The developed biosensor was employed for direct recognition of BSA, where was founded a linear response range between 1.00 x 10(-1) and 1.00 x 10(-6) mg mL(-1) using DPV technique, and the limit of detection calculated of 0.59 x 10(-6) mg mL(-1). Our results indicate that this molecular imprinting onto ITO substrate can be useful as an alternative for bovine serum albumin detection.

Automated summary

This paper presents a method of electrodeposition of a molecularly imprinted polymer onto an indium tin oxide substrate for the determination of bovine serum albumin. Different characterization techniques were used to analyze the fabrication stages of the imprinted polymer films, and the electrochemical properties of the films were investigated. A biosensor was developed for the direct recognition of bovine serum albumin, showing a linear response range and low detection limit.