Investigation of oxygen influence to the optical properties of tirapazamine

New data on 3-amino-1,2,4-benzotriazine 1,4-dioxide (tirapazamine) fluorescence has been obtained using the Perkin-Elmer Lambda 950 UV-Vis-NIR spectrophotometer experimental technique in combination with the extensive DFT-theory approach. Based on the results obtained, we revealed that the optical properties of the molecule under study remain significantly unchanged when the number of oxygen substitutions decreases from 2 to 0. Here we also present the results of the study of the influence of acetonitrile and ethyl acetate on the fluorescence of tirapazamine with the different number of oxygen atoms. Results of our investigation indicate the formation of anion in the case of 3-amino-1,2,4-benzotriazine 1,4-dioxide with two oxygen atoms and their transformation to tirapazamine with one oxygen atom.

Automated summary

New data on the fluorescence of tirapazamine were obtained using experimental and theoretical methods. The optical properties of the molecule were found to remain unchanged when the number of oxygen substitutions decreased. The study also revealed the formation of an anion in the case of tirapazamine with two oxygen atoms and its transformation to tirapazamine with one oxygen atom.