期刊
JOURNAL OF MOLECULAR BIOLOGY
卷 434, 期 14, 页码 -出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2022.167660
关键词
disordered regions; repeat sequences; D; E repeat; polyampholytes; electrostatics
资金
- United States - Israel Binational Science Foundation [2020624]
- National Science Foundation [MCB-2026805]
- Office Of Internatl Science &Engineering
- Office Of The Director [2020624] Funding Source: National Science Foundation
Intrinsically disordered regions (IDRs) of proteins have a high fraction of charged residues, but their overall net charge and organization differ. This study analyzed the charge properties of IDRs in human, mouse, and yeast proteomes to explore the sequence-function relationship. Negatively charged IDRs were found to be longer and have a higher net charge per residue compared to positively charged IDRs. D/E repeats were longer and more prevalent than K/R repeats, especially in nucleic acid-related proteins.
Intrinsically disordered regions (IDRs) of proteins are often characterized by a high fraction of charged residues, but differ in their overall net charge and in the organization of the charged residues. The function-encoding information stored via IDR charge composition and organization remains elusive. Here, we aim to decipher the sequence-function relationship in IDRs by presenting a comprehensive bioinformatic analysis of the charge properties of IDRs in the human, mouse, and yeast proteomes. About 50% of the proteins comprise at least a single IDR, which is either positively or negatively charged. Highly negatively charged IDRs are longer and possess greater net charge per residue compared with highly positively charged IDRs. A striking difference between positively and negatively charged IDRs is the characteristics of the repeated units, specifically, of consecutive Lys or Arg residues (K/R repeats) and Asp or Glu (D/E repeats) residues. D/E repeats are found to be about five times longer than K/R repeats, with the longest found containing 49 residues. Long stretches of consecutive D and E are found to be more prevalent in nucleic acid-related proteins. They are less common in prokaryotes, and in eukaryotes their abundance increases with genome size. The functional role of D/E repeats and the profound differences between them and K/R repeats are discussed.(c) 2022 Elsevier Ltd. All rights reserved.
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