Classification of gases around Pseudomonas aeruginosa and Acinetobacter baumannii by infrared spectroscopy

Gas samples were collected from the air surrounding single and mixed laboratory cultures, and preliminary data on human breath samples were also obtained. The infrared spectra of a variety of gasses were measured at high resolution (0.5 cm(-1)) to obtain information about the infrared absorption bands to be used as indicators. These key bands enable bacterial classification, and the discrimination rates can be improved by observing multiple infrared absorptions. The air around Pseudomonas aeruginosa was distinguished from the other gas samples by the infrared absorptions at wavenumbers of 778.4 cm(-1) and 2213.2 cm(-1). For Acinetobacter baumannii, infrared absorptions at 1215.0 cm-1 and 2982.3 cm(-1) were used; furthermore, adding those at 4768.7 cm(-1) and 5353.8 cm-1 was shown to improve identification.

Automated summary

Gas samples were collected from different laboratory cultures and human breath samples, and their infrared spectra were measured. By observing key infrared absorption bands, bacterial classification and discrimination can be achieved.