期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 195, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.mimet.2022.106449
关键词
Bovine; Brucellosis; ELISA; Sensitivity; Specificity; Diagnostic tests; Serology
资金
- National Dairy Development Board (NDDB)
This study evaluated the fitness of purpose of seven ELISA kits in diagnosing brucellosis among cattle and buffaloes in India. The diagnostic sensitivity and specificity of the kits were determined using different approaches, and their suitability for diagnostic application in various epidemiological situations was discussed.
Seven ELISA kits were evaluated for the fitness of purpose in diagnosing brucellosis among cattle and buffaloes in the endemic scenarios of India. The sera (675 numbers) for the study were sourced from brucellosis-free as well as infected herds. The diagnostic sensitivity (dsn) and specificity (dsp) of the kits were determined by three approaches: based on the results of the Rose Bengal test, history of the animals (sera from infected or nave animals), and based on the results obtained from the 'majority of the tests'. The dsn and dsp ranged from 65.10% to 98.66%, and 98.04% to 100% respectively. The results and suitability of the kits for diagnostic application in various epidemiological situations were discussed.
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