期刊
JOURNAL OF MEDICAL MICROBIOLOGY
卷 71, 期 4, 页码 -出版社
MICROBIOLOGY SOC
DOI: 10.1099/jmm.0.001519
关键词
COVID-19; dipstick; reverse transcription helicase-dependent amplification; SARS-CoV-2
类别
资金
- COVID-19 Rapid Response Grants - Global Infectious Diseases Institute, University of Virginia, Charlottes-ville, Virginia, USA
This study reports a point-of-care detection technique for COVID-19 that combines isothermal amplification and dipstick technologies, providing a simple and rapid diagnostic test that can be performed in low-resource settings.
Early detection of coronavirus disease 2019 (COVID-19) is critical for both initiating appropriate treatment and preventing the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent. A simple and rapid diagnostic test that can be performed without any expensive equipment would be valuable for clinicians working in a low-resource setting. Here, we report a point-of-care detection technique for COVID-19 that combines the power of isothermal amplification (reverse transcription helicase-dependent amplification, RT-HDA) and dipstick technologies. The limit of detection of this diagnostic test is six copies of SARS-CoV-2 mu l(-1) in clinical specimens. Of the 22 clinical specimens tested, RT-HDA-coupled dipstick correctly identified all positive and negative specimens. The RT-HDA can be performed over a heating block and the results can be interpreted visually with the dipstick technology without any specialized equipment. Furthermore, the RT-HDA-coupled dipstick could be performed in a short turnaround time of similar to 2 h. Thus, the RT-HDA-coupled dipstick could serve as a point-of-care diagnostic test for COVID-19 in a low-resource environment.
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