期刊
JOURNAL OF MEDICAL MICROBIOLOGY
卷 71, 期 5, 页码 -出版社
MICROBIOLOGY SOC
DOI: 10.1099/jmm.0.001522
关键词
diagnostics; AMR; LAMP; nucleic acid detection
类别
资金
- European Union [773830]
- WorldCOM project
- Liphook Equine Hospital/Vet Partners Ltd
- Biotechnology and Biological Sciences Research Council (BBSRC)
- Innovate UK
LAMP is a rapid alternative to PCR, allowing the reaction to occur at a single temperature using a polymerase with high displacement activity. Its advantages include the ability to rapidly detect nucleic acid targets within 30 minutes and its ease of use, making it suitable for various diagnostic settings.
Loop-mediated isothermal amplification (LAMP) is a rapid alternative to PCR, in which the reaction occurs at one temperature and uses a polymerase with high displacement activity, e.g. Bacillus stearothermophilus DNA polymerase I (Bst) or homologues. Since the discovery of LAMP in 2000, several applications have been developed to employ this technique in the rapid detection of nucleic acid targets and enhance its performance. Improvements to the LAMP technique and a variety of innovative detection methods have led to its application for a wide range of targets in medical and veterinary microbiology, particularly in resource-poor settings. The key advantages of LAMP-based diagnostics include the ability to rapidly detect target nucleic acid sequences within 30 min and its ease of use, facilitating its application in field, bedside, pen-side, point-of-care and point-of-need diagnostic settings. LAMP can be a valuable tool to aid in the detection and management of disease outbreaks.
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