Dual-functional bacterial cellulose modified with phase-transitioned proteins and gold nanorods combining antifouling and photothermal bactericidal properties

Bacterial cellulose (BC) is one of the most versatile natural biopolymers with unique physical, chemical, and biological features. However, the lack of intrinsic antibacterial property of native BC limits its broad biomedical applications where such property is highly required to prevent contamination or infection caused by attached bacteria. In this work, we developed a simple and facile method to fabricate a dual functional BC membrane by physical incorporation of gold nanorods (GNRs) followed by deposition of a phase-transitioned bovine serum albumin (PTB) film. Due to the broad-spectrum antifouling property of the PTB film, the resulting membrane could prevent the adhesion and accumulation of bacteria. A few bacteria that broke through the protection of the PTB film could be eradicated under short-term irradiation of a near-infrared laser due to the excellent photothermal property of incorporated GNRs. The whole fabrication was conducted in a simple and environmentally friendly manner, avoiding complicated processes and toxic organic solvents. Moreover, because all the components were biocompatible, the resulting membrane showed negligible cytotoxicity in vitro and good histocompatibility in vivo . This work thus provided a reliable way to endow BC with antibacterial property, being beneficial for diverse biomedical applications. (c) 2021 Published by Elsevier Ltd on behalf of Chinese Society for Metals.

Automated summary

This study presents a simple method to fabricate a dual functional bacterial cellulose membrane that can prevent bacterial adhesion and accumulation, and eradicate bacteria under near-infrared laser irradiation. The fabrication process is simple, environmentally friendly, and the resulting membrane exhibits good biocompatibility.