期刊
JOURNAL OF INVERTEBRATE PATHOLOGY
卷 189, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2022.107726
关键词
Pinewood nematode; Bacillus thuringiensis; Cry toxins; Nematocidal activity; Intestinal damage
类别
资金
- National Major Emergency Science and Technology Program of China [ZD202001]
- National Key R & D Program of China [2021YFD1400900]
- Forestry Key Program of Science and Technology in Fujian Province [2021FKJ03]
- National Natural Science Foundation of China [U1905201, 32171805]
- Science Fund for Distinguished Young Scholars of Fujian Agriculture and Forestry Uni-versity [xjq201614]
- Forestry Programs of Science and Technology in Fujian Province [[2020] 601]
- China Postdoctoral Science Foundation [2017M612107]
- Science and Technology Program of Fujian Province [2018N5002]
- Forestry Science Research Project of Fujian Forestry Department [[2017] 03]
- China Scholarship Council [202008350145]
- [Mincaizhi [2020] 601]
The pine wilt disease caused by Bursaphelenchus xylophilus results in significant ecological and economic losses. This study evaluated the nematocidal activity of various toxins produced by Bacillus thuringiensis against B. xylophilus. Six toxins were identified to have high toxicity, leading to the shrinkage, thinning, and degeneration of the pinewood nematodes. These findings provide valuable insights into the action mechanism of nematocidal toxins and their potential use in the biological control of B. xylophilus.
The pine wilt disease is caused by the pinewood nematode Bursaphelenchus xylophilus and it results in serious ecological and economic losses. Therefore, effective prevention and control methods for the pinewood nematode are urgently required. Bacillus thuringiensis (Bt), a widely used microbial insecticide, produces toxins that are toxic to several species of parasitic nematodes, however, its effects on B. xylophilus have not been determined. In this study, Cry5Ba3, App6Aa2, Cry12Aa1, Cry13Aa1, Cry14Aa1, Cry21Aa3, Cry21Fa1, Xpp55Aa1, and Cyt8Aa1 toxins' nematocidal activity against B. xylophilus was evaluated, six toxins with high toxicity were identified: App6Aa2 (LC50 = 49.71 mu g/mL), Cry13Aa1 (LC50 = 53.17 mu g/mL), Cry12Aa1 (LC50 = 58.88 mu g/mL), Cry5Ba3 (LC50 = 63.99 mu g/mL), Xpp55Aa1 (LC50 = 65.14 mu g/mL), and Cyt8Aa1 (LC50 = 96.50 mu g/mL). The six toxins caused shrinkage and thinning of the intestinal cells, contraction of the intestine from the body wall, vacuolization, and degenerated appearance of the pinewood nematodes. The results of this study provide basic information to study the action mechanism of nematocidal toxins on the pinewood nematode and direction for the use of nematocidal toxins in the biological control of B. xylophilus.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据