期刊
JOURNAL OF INFECTIOUS DISEASES
卷 226, 期 3, 页码 528-540出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiac112
关键词
African trypanosomes; vaccination; memory B cells; adaptive immunity
资金
- Ghent University Global Campus core funding
- Ghent University BOF grant [STG.2018.0009.01/01N01518]
- FWO (Fonds voor Wetenschappelijk Onderzoek Vlaanderen) [G013518N]
- R&D Department of the Vrije Universiteit Brussel [SRP63]
This study demonstrates that trypanosomosis induces general immunological B-cell memory loss, benefiting the parasite by reducing the requirements for surface antigenic variation.
Using 2 independent vaccination strategies, this study showed that trypanosomosis induces general immunological B-cell memory loss, which benefits the parasite by reducing the stringency for surface antigenic variation. Background Trypanosoma brucei brucei evades host immune responses by multiple means, including the disruption of B-cell homeostasis. This hampers anti-trypanosome vaccine development. Because the cellular mechanism underlying this pathology has never been addressed, our study focuses on the fate of memory B cells (MBCs) in vaccinated mice upon trypanosome challenge. Methods A trypanosome variant surface glycoprotein (VSG) and fluorescent phycoerythrin were used as immunization antigens. Functional and cellular characteristics of antigen-specific MBCs were studied after homologous and heterologous parasite challenge. Results Immunization with AnTat1.1 VSG triggers a specific antibody response and isotype-switched CD73(+)CD273(+)CD80(+) MBCs, delivering 90% sterile protection against a homologous parasite challenge. As expected, AnTat1.1 VSG immunization does not protect against infection with heterologous VSG-switched parasites. After successful curative drug treatment, mice were shown to have completely lost their previously induced protective immunity against the homologous parasites, coinciding with the loss of vaccine-induced MBCs. A phycoerythrin immunization approach confirmed that trypanosome infections cause the general loss of antigen-specific splenic and bone marrow MBCs and a reduction in antigen-specific immunoglobulin G. Conclusions Trypanosomosis induces general immunological memory loss. This benefits the parasites by reducing the stringency for antigenic variation requirements.
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