Unraveling the plastic degradation potentials of the plastisphere-associated marine bacterial consortium as a key player for the low-density polyethylene degradation

The omnipresent accumulation and non-degradable nature of plastics in the environment are posing an everincreasing ecological threat. In this study, a total of 97 bacteria were isolated from macroplastic debris collected from the coastal environments of Andaman Island. The isolates were screened for LDPE degradation potential and were identified based on phenotypic, biochemical, and molecular characterization. 16S rDNAbased identification revealed that three-three isolates of each belong to the genus Oceanimonas and Vibrio, two were closely related to the genus Paenibacillus whereas, one-one was associated with the genus Shewanella, Rheinheimera, and Bacillus, respectively. A bacterial consortium was formulated using the top four isolates based on their individual LDPE degradation potentials. A significant increase (p < 0.05) in the mean LDPE degradation (47.07 +/- 6.67% weight-loss) and change in thickness was observed after 120 days of incubation. FTIR spectrum, 13C NMR, and TG-DSC analyses demonstrated changes in the LDPE sheets' functional groups, crystallinity, and in thermal properties after 120 days of incubation. The SEM and AFM images confirmed bacterial attachments, an increase in surface roughness and deformities on LDPE sheets. This study reports a bacterial consortium that can efficiently degrade the plastics and can be used in providing eco-friendly mitigation of plastic waste.

Automated summary

This study isolated 97 bacteria with LDPE degradation potential from macroplastic debris collected from Andaman Island, and formulated a bacterial consortium that efficiently degrades plastics. Incubation experiments confirmed significant increase in LDPE degradation by the consortium, demonstrating its potential as an eco-friendly solution for plastic waste mitigation.