期刊
JOURNAL OF GENERAL VIROLOGY
卷 103, 期 4, 页码 -出版社
MICROBIOLOGY SOC
DOI: 10.1099/jgv.0.001735
关键词
SARS- CoV-2; COVID-19; coronavirus; sarbecovirus; host range; zoonosis
资金
- G2P-UK National Virology Consortium - MRC [G2P-UK]
- National Virology Consortium to address phenotypic consequences of SARS-CoV-2 genomic variation [MR/W005611/1]
- Pirbright Institute's BBSRC institute strategic programme grant [BBS/E/I/00007038, BBS/E/I/00007034, BB/T008784/1]
- MRC [MR/V036750/1]
- MRC [MR/V036750/1] Funding Source: UKRI
This study investigated the host ACE2 receptor usage patterns of alpha, beta, gamma, and delta variants using viral pseudotyping, and found that these variants have different characteristics in different hosts.
Following the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV- 2) in PR China in late 2019 a number of variants have emerged, with two of these - alpha and delta - subsequently growing to global prevalence. One characteristic of these variants are changes within the spike protein, in particular the receptor-binding domain (RBD). From a public health perspective, these changes have important implications for increased transmissibility and immune escape; however, their presence could also modify the intrinsic host range of the virus. Using viral pseudotyping, we examined whether the variants of concern (VOCs) alpha, beta, gamma and delta have differing host angiotensin-converting enzyme 2 (ACE2) receptor usage patterns, focusing on a range of relevant mammalian ACE2 proteins. All four VOCs were able to overcome a previous restriction for mouse ACE2, with demonstrable differences also seen for individual VOCs with rat, ferret or civet ACE2 receptors, changes that we subsequently attributed to N501Y and E484K substitutions within the spike RBD.
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