期刊
JOURNAL OF FOOD PROTECTION
卷 85, 期 5, 页码 755-772出版社
ELSEVIER
DOI: 10.4315/JFP-21-437
关键词
Foodborne outbreak; Food safety; Molecular subtyping; US public health agencies; Whole genome sequencing
资金
- National Library of Medicine at NIH
This report provides an overview of the use of whole genome sequencing (WGS) technology for detecting and characterizing foodborne pathogens and identifying their sources. It highlights the collaborative efforts among federal agencies in food safety and describes the methods used in genetic analysis networks. The report emphasizes the application of WGS in pathogen characterization and source attribution, as well as the impact of culture-independent diagnostic tests on food safety analysis.
This multiagency report developed by the Interagency Collaboration for Genomics for Food and Feed Safety provides an overview of the use of and transition to whole genome sequencing (WGS) technology for detection and characterization of pathogens transmitted commonly by food and for identification of their sources. We describe foodbome pathogen analysis, investigation, and harmonization efforts among the following federal agencies: National Institutes of Health; Department of Health and Human Services, Centers for Disease Control and Prevention (CDC) and U.S. Food and Drug Administration (FDA); and the U.S. Department of Agriculture, Food Safety and Inspection Service, Agricultural Research Service, and Animal and Plant Health Inspection Service. We describe single nucleotide polymorphism, core-genome, and whole genome tnultilocus sequence typing data analysis methods as used in the PulseNet (CDC) and GenomeTrakr (FDA) networks, underscoring the complementary nature of the results for linking genetically related foodbome pathogens during outbreak investigations while allowing flexibility to meet the specific needs of Interagency Collaboration partners. We highlight how we apply WGS to pathogen characterization (virulence and antimicrobial resistance profiles) and source attribution efforts and increase transparency by making the sequences and other data publicly available through the National Center for Biotechnology Information. We also highlight the impact of current trends in the use of culture-independent diagnostic tests for human diagnostic testing on analytical approaches related to food safety and what is next for the use of WGS in the area of food safety.
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