4.6 Article

Proteomic characterization of pistachio nut allergen proteins

期刊

出版社

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jfca.2021.104337

关键词

Allergens; Pistachio nut; Quantitative proteomics; SDS-PAGE; Shotgun proteomics; Two-dimensional electrophoresis

资金

  1. project Safe & Smart Nuove tecnologie abilitanti per la food safety e l'integrit`a delle filiere agroalimentari in uno scenario globale e National CL.AN Cluster agroalimentare nazionale programma area 2 [MIUR CTN01_00230_248064]

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In this study, the proteome composition of pistachio seeds was investigated using various proteomics analyses. The results identified proteins belonging to the cupin protein family and allergens, and also compared the proteomes of different pistachio varieties.
Pistachio nut (Pistacia vera) is an important tree nut cultivated widely worldwide and is commonly appreciated based on its organoleptic characteristics and nutritional healthfulness. Similar to other tree nuts, the consumption of pistachio nuts is associated with moderate or severe allergic reactions in sensitized/allergic individuals. In the present study, we investigated the proteome composition of pistachio seeds using gel-based (mono- and two-dimensional electrophoresis) and gel-free (shotgun and label free quantitative) proteomics analyses. The gel-based approach allowed the identification of proteins belonging to the cupin protein family (11S and 7S globulins) and suggested the occurrence of multiple isoforms. The shotgun proteomic analysis extended the identification to allergens 25 albumin and heat shock protein, whereas label free quantitative was employed to investigate the proteomes of four different pistachio varieties. Protein pattern characterization through sophisticated techniques, such as those developed by proteomics science, allowed the identification of distinct protein markers on different pistachio cultivars.

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