期刊
JOURNAL OF ENVIRONMENTAL ENGINEERING
卷 148, 期 4, 页码 -出版社
ASCE-AMER SOC CIVIL ENGINEERS
DOI: 10.1061/(ASCE)EE.1943-7870.0001978
关键词
Anaerobic digestion; Microbial taxonomy; Cellulose
资金
- Environmental Research & Education Foundation, Raleigh, North Carolina
This study investigated the taxonomies of bacteria, archaea, and fungi in a cellulolytic enrichment reactor using sequencing techniques. The study also demonstrated the use of a centrifugation separation technique to analyze different biomass fractions. The results showed significant differences in taxonomic compositions between the sessile and planktonic biomass fractions.
The bacterial, archaeal, and fungal taxonomies of a semi-batch-fed, lab-scale cellulolytic enrichment reactor inoculated by the organic fraction of municipal solid waste were explored using 16SrRNA and internal transcribed spacer (ITS) sequencing. Biomass samples were partitioned into total, planktonic, and sessile (biofilm) fractions using a centrifugation separation technique developed to quantify sessile biomass growth on cellulose particles during anaerobic digestion. The relative abundances of bacteria and archaea taxa were determined over a 1-day period. The sessile and planktonic biomass fractions showed significantly different relative abundances for 13 taxa, with the sessile biomass having 10 times lower relative abundance of Unclassified Firmicutes, nearly 20 times higher relative abundance of Methanothrix spp., double the relative abundance of Hydrogenispora spp., and 44.3% higher relative abundance of an uncultured Leptospira sp. than the planktonic biomass. This study shows how a relatively simple physical separation method can be employed to add another dimension to taxonomic studies. Better understanding the microbial communities in these systems is a key first step toward achieving performance improvements such as enhanced cellulose solubilization rates and other microbial biotransformations through process, genetic, and/or other manipulations.
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