Comparison of two pyrolytic graphite representatives in the construction of hybrid electrochemical DNA biosensors for monitoring DNA damage

Electrochemical DNA biosensors represent a variety of versatile analytical tools successfully used in many important applications. Their utilization in the area of monitoring DNA damage is considered as the prominent field that can provide us with an improved general knowledge regarding DNA damaging events occurring in vivo by an unorthodox overall insight employing the various electrochemical methods. Herein, we present development, optimization process, and subsequent analytical testing of the novel hybrid electrochemical DNA biosensors based on the two representatives of the pyrolytic graphite - basal-plane pyrolytic graphite (BPPG) and edge-plane pyrolytic graphite (EPPG) - and low-molecular-weight double-stranded DNA (dsDNA). Closer resolution of the nature of the oxidation/reduction signals of the dsDNA, as well as the most important optimization of parameters securing satisfying operational stability of the proposed hybrid biosen-sors, is investigated. Subsequent analytical testing of the novel hybrid biosensors and the evaluation of their advantages/disadvantages in terms of monitoring DNA damage caused by an UV light irradiation employing various voltammetric methods (square wave (SWV), linear sweep (LSV), or cyclic (CV) voltammetry) and elec-trochemical impedance spectroscopy (EIS) is also presented.

Automated summary

Electrochemical DNA biosensors have been widely used in various applications, and their application in monitoring DNA damage is particularly significant. A novel hybrid electrochemical DNA biosensor based on graphene was developed and optimized, and its performance in monitoring DNA damage was evaluated. The results showed that the biosensor has important advantages in monitoring DNA damage.