4.5 Article

Octaarginine functionalized nanoencapsulated system: In vitro and in vivo evaluation of bFGF loaded formulation for wound healing

出版社

ELSEVIER
DOI: 10.1016/j.jddst.2022.103343

关键词

Octaarginine; Chitosan; Nanoencapsulation; Wound healing; Fibroblast growth factor; In vivo protein delivery

资金

  1. Izmir Katip Celebi University, Scientific Research Projects Coordinatorship [2019-ONAP-ECZF-0002]

向作者/读者索取更多资源

An innovative protein delivery method was developed in this study, where basic fibroblast growth factor was nanoencapsulated into a gel system and combined with cell penetrating peptide octaarginine, effectively accelerating the healing of diabetic wounds.
Despite all treatment efforts, diabetic wounds are still a major health concern that needs to be solved. Growth factor-based therapeutic approaches offer promising results for wound healing therapy. However, their high sensitivity to proteolysis causes repetitive application, and associated high cost makes it necessary to develop an effective delivery system. In this study, an innovative approach for protein delivery was developed and evaluated in terms of wound healing activity. For this purpose, basic fibroblast growth factor was first encapsulated into chitosan and alginate based polymeric system. Then, a cell penetrating peptide, octaarginine, was incorporated onto the system to achieve a higher therapeutic effect. The characterization studies were performed, and the optimal nanoparticle system was dispersed in hydroxypropyl methylcellulose for providing skin application. The in vitro and in vivo efficiency of the system was also evaluated. According to the results, the obtained gel formulation is safe for the applied doses up to 150 mg/mL and significantly promotes fibroblast cell migration in 48 h. Furthermore, in vivo studies revealed that the diabetic wound healing efficiency of basic fibroblast growth factor nanoencapsulated gel system significantly accelerates wound healing. Moreover, by functionalizing this system with octaarginine, epithelization, granulation of tissue formation, and treatment efficacy were promoted. In conclusion, this work designs a suitable cell penetrating peptide functionalized nanoencapsulated system for growth factor delivery, which provides a promising therapeutic potential for hard-to-heal skin lesions, particu-larly diabetic wounds.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据