期刊
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
卷 71, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jddst.2022.103340
关键词
Nanocomposite; Controlled cytotoxicity; Green synthesis; Graphene oxide; Silver nanoparticles
资金
- Research and Technology Institute of Plant Production, Shahid Bahonar University of Kerman, Kerman, Iran [P/00/156]
This study developed a green approach to fabricate silver nanoparticles and graphene oxide-silver nanocomposites, and evaluated their biological activities against cancerous and normal cells. The results showed that both nanomaterials exhibited higher activity against cancer cells, with silver nanoparticles being more cytotoxic.
The lack of definitive treatment for cancer and the emergence of drug-resistant cancers highlights the need for alternative therapies and effective anti-cancer agents. Here, we developed and applied a green approach to fabricate Silver nanoparticles (AgNPs) and Graphene oxide-Silver nanocomposite (Ag-GO) using the black peel pomegranate extract. The nanomaterials' physicochemical properties were characterized using UV-Visible, FTIR, TEM-EDX, and Zeta potential analyses. Then, the nanomaterials' biological activities against cancerous (MCF-7) and normal (HUVEC) cells were evaluated via MTT assay, real-time PCR, and ROS/DCFDA assay protocols. Based on the results, synthesized AgNPs were spherical with 35 +/- 5 nm diameter and adequate dispersity. However, very small and uniform AgNPs (10 +/- 2 nm) with great dispersity were formed on the GO surface. Also, the nanocomposite surface charge (-41 mV) was substantially lower than that of AgNPs (-28 mV), indicating higher stability. Both AgNPs and Ag-GO showed much more activity against cancerous cells than normal ones. Yet, AgNPs cytotoxicity was severe, so that 500 mu g/ml of this compound, independently of time, reduced the viability of HUVEC and MCF-7 cells to about 41% and 20%, respectively. Ideally, the Ag-GO activity was moderate and time-dependent. 24 h exposure of MCF-7 cells to this compound (500 mu g/ml) led to the increase of cell death from 30% to more than 50% after 48 h. Both nanocompounds increased the Bax/Bcl-2 ratio and intracellular ROS, though, AgNPs showed the most activity to increase the Bax/Bcl-2 ratio, while Ag-GO more effectively increased the amount of ROS levels. These findings demonstrated the method's capability of synthesizing nanocomposites with desirable physicochemical and biological properties.
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