期刊
JOURNAL OF CLINICAL IMMUNOLOGY
卷 42, 期 6, 页码 1254-1269出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10875-022-01291-9
关键词
Immunodeficiency; T cells; CVID; Exhaustion; Activation; Differentiation
类别
资金
- Deutsche Forschungsgemeinschaft [SFB 1160 IMPATH]
- European Society for Immunodeficiencies
- Czech Health Research Council
- Ministry of Health, Czech Republic [AZV NV18-05-00162, NU20-05-00282]
- Projekt DEAL
By analyzing CD8 T cell homeostasis in patients with CVID, it was found that these cells undergo advanced differentiation, exhaustion, activation, and immunoregulatory processes. Assessing the phenotype of CD8 T cells can help predict the risk of CVID patients and provide a better understanding of the pathogenesis of CVID, including T cell exhaustion and regulation mechanisms.
Purpose Common variable immunodeficiency (CVID) is the most frequent symptomatic primary immunodeficiency, with heterogeneous clinical presentation. Our goal was to analyze CD8 T cell homeostasis in patients with infection only CVID, compared to those additionally affected by dysregulatory and autoimmune phenomena. Methods We used flow and mass cytometry evaluation of peripheral blood of 40 patients with CVID and 17 healthy donors. Results CD8 T cells are skewed in patients with CVID, with loss of naive and increase of effector memory stages, expansion of cell clusters with high functional exhaustion scores, and a highly activated population of cells with immunoregulatory features, producing IL-10. These findings correlate to clinically widely used B cell-based EURO classification. Features of exhaustion, including loss of CD127 and CD28, and expression of TIGIT and PD-1 in CD8 T cells are strongly associated with interstitial lung disease and autoimmune cytopenias, whereas CD8 T cell activation with elevated HLA-DR and CD38 expression predict non-infectious diarrhea. Conclusion We demonstrate features of advanced differentiation, exhaustion, activation, and immunoregulatory capabilities within CD8 T cells of CVID patients. Assessment of CD8 T cell phenotype may allow risk assessment of CVID patients and provide new insights into CVID pathogenesis, including a better understanding of mechanisms underlying T cell exhaustion and regulation.
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