A hyperbranched polyglycerol-functionalized polymer polar stationary phase

A polymer polar stationary phase functionalized with hyperbranched polyglycerol for hydrophilic interac-tion chromatography (HILIC) is described. It is prepared via surface-initiated ring-opening polymerization of hyperbranched polyglycerol onto hydrolzed poly(glycidyl methacrylate-divinylbenzene) microspheres. The capacity of the functional groups can be maniputed by repeating hyperbranch layers. The phase showed typical HILIC character with good separation performance towards tested polar analytes. It also exhibited wider pH tolerance range (e.g. at least 2 to 12) and as well negligible bleed level under gradient elution mode (even to 50% fraction of water).(c) 2022 Elsevier B.V. All rights reserved.

Automated summary

This study describes a polymer polar stationary phase functionalized with hyperbranched polyglycerol for hydrophilic interaction chromatography (HILIC). The functionalized phase demonstrates good separation performance, wider pH tolerance range, and minimal bleed level under gradient elution mode.