期刊
JOURNAL OF CELLULAR PHYSIOLOGY
卷 237, 期 5, 页码 2528-2538出版社
WILEY
DOI: 10.1002/jcp.30718
关键词
bone morphogenetic proteins; fertility; fibroblast growth factors; ovary
资金
- Natural Science and Engineering Research Council (NSERC) Canada
- Agence Nationale pour la Recherche France [ANR-12-BSV1-0034-02]
- European Funds for Regional Development (FEDER) through the Interreg POCTEFA programme [EFA103/15]
- Fonds de recherche du Quebec-Nature et technologies/Reseau Quebecois en Reproduction
- Agence Nationale de la Recherche (ANR) [ANR-12-BSV1-0034] Funding Source: Agence Nationale de la Recherche (ANR)
Increasing the efficiency of farm animal reproduction is important for reducing the environmental impact of food production systems. This study investigates the factors that decrease oocyte health and how fibroblast growth factor 18 (FGF18) is regulated in the ovarian follicle. The researchers found that FGF18 secretion is controlled by intrafollicular factors and is linked to fertility. They also discovered that BMP4 stimulates FGF18 secretion from thecal endothelial cells, which affects fertility. Understanding these processes can help improve reproductive efficiency in farm animals.
Increasing the efficiency of farm animal reproduction is necessary to reduce the environmental impact of food production systems. One approach is to increase the number of healthy eggs (oocytes) produced per female for fertilization, thus it is important to understand factors that decrease oocyte health. One paracrine factor that decreases ovarian follicle growth is fibroblast growth factor 18 (FGF18) secreted by cells in the theca layer of the ovarian follicle, however the factors that regulate FGF18 secretion are unknown. In this study we hypothesized that FGF18 secretion is controled by intrafollicular factors and is linked to fertility, which we tested by using cell culture and sheep genetic models in vivo. Separation of theca cell populations revealed that FGF18 messenger RNA (mRNA) is located mainly in thecal endothelial rather than endocrine cells, and immunohistochemistry localized FGF18 protein to microvessels in the theca layer in situ. Culture of ovine theca-derived endothelial cells was used to demonstrate stimulation of FGF18 mRNA and protein abundance by bone morphogenetic protein 4 (BMP4), a growth factor derived from theca endocrine cells. Taking advantage of a sheep genetic model, we demonstrate reduced ovarian and peripheral FGF18 concentrations in the hyperprolific Booroola ewe harboring the FecB(B) mutation in BMPR1B. These data suggest a novel control of fertility by follicular endothelial cells, in which theca endocrine cells secrete BMP4 that stimulates the secretion of FGF18 from thecal endothelial cells, which in turn diffuses into the granulosa cell layer and promotes apoptosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据