4.5 Article

Towards commercial levels of astaxanthin production in Phaffia rhodozyma

期刊

JOURNAL OF BIOTECHNOLOGY
卷 350, 期 -, 页码 42-54

出版社

ELSEVIER
DOI: 10.1016/j.jbiotec.2022.04.001

关键词

Phaffia rhodozyma (also known as Xanthophyllomyces dendrorhous ); Astaxanthin; Next generation sequencing; Kinetic model; Surface response; Random mutagenesis

资金

  1. Queensland Government (Australia) , Queensland University of Technology (Australia) [AQKTP00116-17RD2]
  2. Bioproton Pty Ltd (Australia) through the Advance Queensland [AQIRF167-2018RD1]
  3. Advance Queensland Industry Research Fellowship
  4. Queensland Government (Australia)
  5. Queensland University of Technology (Australia)
  6. Bioproton Pty Ltd (Australia) through the Advance Queensland Knowledge Transfer Partnership [AQKTP00116-17RD2]
  7. Advance Queensland Industry Research Fellowship programs [AQIRF167-2018RD1]

向作者/读者索取更多资源

This study found that mutant strains of P. rhodozyma generated through mutagenesis showed increased production of astaxanthin (AX). Culture media optimization and kinetic modeling were used to maximize AX production. Genomic variant analysis revealed important mutations in protein coding regions associated with regulators and catalysts of AX production.
Astaxanthin (AX) is a potent antioxidant with increasing biotechnological and commercial potential as a feed supplement, and gives salmonids and crustaceans their attractive characteristic pink color. The red yeast Phaffia rhodozyma naturally produces AX as its main fermentation product but wild-type strains and those previously generated through classical random mutagenesis produce low yields of AX. Existing strains do not meet commercial economic requirements, fundamentally due to a lack of understanding of the underlying mechanisms and genotype-phenotype associations regarding AX production in P. rhodozyma. In the present study, screening of P. rhodozyma CBS 6938 mutant strains generated through chemical and ultra violet radiation mutagenesis delivered increased AX production yields that were then maximized using culture media optimization and fed batch culture kinetic modeling. The whole genomes of the wild-type and eight increased production strains were sequenced to identify genomic changes. The selected strains produced 50-fold more AX than the wild-type strain with a total biomass of around 100 gDCW/L and a carotenoid production of 1 g/L. Genomic variant analyses found 368 conserved mutations across the selected strains with important mutations found in protein coding regions associated with regulators and catalysts of AX precursors in the mevalonate pathway, the electron transport chain, oxidative stress mechanisms, and carotenogenesis.

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