Quantification of leuco-indigo in indigo-dye-fermenting suspension by normal pulse voltammetry

Article Biochemistry & Molecular Biology

Isolation and characterization of indigo-reducing bacteria and analysis of microbiota from indigo fermentation suspensions

Kasumi Nakagawa et al.

Summary: In this study, we isolated and identified microorganisms involved in the reduction of indigo and analyzed the microbiota in the indigo-fermentation suspensions. We found that anthraquinone is an effective mediator for the indigo-reduction activity of the isolated strains, and maintaining the ratio of indigo-reducing bacteria is crucial for the fermentation process.

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY (2022)

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Article Electrochemistry

Mechanistic Insights into Indigo Reduction in Indigo Fermentation: A Voltammetric Study

Kasumi Nakagawa et al.

Summary: Indigo, one of the oldest natural blue dyes, is deeply involved in traditional staining with the help of microorganisms and their enzymatic activities. The mechanism of microbial indigo reduction was elucidated by observing redox peaks of leuco-indigo and the two-way microbially catalyzed oxidation and reduction. Acetaldehyde was found to be the electron donor and acceptor in the catalytic reactions.

ELECTROCHEMISTRY (2021)

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Article Biotechnology & Applied Microbiology

Voltammetric in-situ monitoring of leuco-indigo in indigo-fermenting suspensions

Kasumi Nakagawa et al.

Summary: This study successfully applied cyclic voltammetry to in-vivo monitoring of leuco-indigo in indigo-fermenting suspensions, revealing no need for deoxygenation under quiescent conditions. The anodic peak current was used as a measure of leuco-indigo concentration. The method, which does not require electrode surface pretreatment, can reflect the dyeing activity of suspensions and clarify aspects such as the number of days required for dye fermentation and the importance of specific nutrient additions.

JOURNAL OF BIOSCIENCE AND BIOENGINEERING (2021)

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Article Chemistry, Physical

Indigo-Mediated Semi-Microbial Biofuel Cell Using an Indigo-Dye Fermenting Suspension

Mayu Kikuchi et al.

Summary: This study successfully constructed a semi-microbial biofuel cell using an indigo-dye fermenting suspension, demonstrating high open-circuit voltage and output power. In terms of stability, the anode exhibited high stability initially but showed a slight decrease in voltage over time.

CATALYSTS (2021)

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Review Microbiology