期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
卷 110, 期 8, 页码 1968-1990出版社
WILEY
DOI: 10.1002/jbm.b.35034
关键词
chemically defined; human pluripotent stem cells; peptide; synthetic surface; three-dimensional culture
资金
- Chengguan District Science and Technology Project [2018-7-6]
- Fundamental Research Funds for the Central Universities [lzujbkj-2019-ct07, lzujbky-2021-ey14, lzujbky2021-kb05]
- Gansu Provincial Natural Science Foundation [20JR5RA253]
- Lanzhou University Hospital of Stomatology Research Support Fund [LZUKQKY-2019-Y10, lzukqky-2019-t9]
- National Natural Science Foundation of China [81801855]
This review summarizes the design and limitations of reported synthetic substrates for cell culture, focusing on the development of promising peptide displaying surfaces and the biophysical regulation of synthetic substrate surfaces as well as three-dimensional culture systems.
Human pluripotent stem cells (hPSCs) have the potential of long-term self-renewal and differentiation into nearly all cell types in vitro. Prior to the downstream applications, the design of chemically defined synthetic substrates for the large-scale proliferation of quality-controlled hPSCs is critical. Although great achievements have been made, Matrigel and recombinant proteins are still widely used in the fundamental research and clinical applications. Therefore, much effort is still needed to improve the performance of synthetic substrates in the culture of hPSCs, realizing their commercial applications. In this review, we summarized the design of reported synthetic substrates and especially their limitations in terms of cell culture. Moreover, much attention was paid to the development of promising peptide displaying surfaces. Besides, the biophysical regulation of synthetic substrate surfaces as well as the three-dimensional culture systems were described.
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