期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 298, 期 7, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jbc.2022.102077
关键词
-
资金
- NIH [R01GM065204, R01HD096745]
- Yale School of Medicine
This study reveals the important role of TXNRD3, a member of the thioredoxin reductase family, in sperm development and maturation. The deficiency of TXNRD3 impairs sperm morphology and motility, as well as mitochondrial ultrastructure and activity.
During epididymal transit, redox remodeling protects mammalian spermatozoa, preparing them for survival in the subsequent journey to fertilization. However, molecular mechanisms of redox regulation in sperm development and maturation remain largely elusive. In this study, we report that thioredoxin-glutathione reductase (TXNRD3), a thioredoxin reductase family member particularly abundant in elongating spermatids at the site of mitochondrial sheath formation, regulates redox homeostasis to support male fertility. Using Txnrd3(-/-) mice, our biochemical, ultrastructural, and live cell imaging analyses revealed impairments in sperm morphology and motility under conditions of TXNRD3 deficiency. We find that mitochondria develop more defined cristae during capacitation in wildtype sperm. Furthermore, we show that absence of TXNRD3 alters thiol redox status in both the head and tail during sperm maturation and capacitation, resulting in defective mitochondrial ultrastructure and activity under capacitating conditions. These findings provide insights into molecular mechanisms of redox homeostasis and bioenergetics during sperm maturation, capacitation, and fertilization.
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