Two previously undescribed phthalides from Talaromyces amestolkiae, a symbiotic fungus of Syngnathus acus

Amestolkins A (1) and B (2), two previously undescribed phthalides sharing the same planar structure of (1, 5-dihydroxyhexyl)-7-hydroxyisobenzofuran-1(3H)-one were isolated from Talaromyces amestolkiae. Their absolute configurations were elucidated by comprehensive analyses of spectroscopic evidences in high-resolution electrospray mass spectra (HRESIMS) and nuclear magnetic resonance (NMR) combined with electronic circular dichroism (ECD) and NMR calculations. 1 and 2 showed anti-neuroinflammatory activity by inhibiting the gene expressions of proinflammatory factors including C-C motif chemokine ligand 2 (CCL-2), tumor necrosis factor-alpha (TNF-alpha) and interleukin 6 (IL-6), as well as attenuating the excretion of inducible nitric oxide synthase (iNOS) in BV-2 microglial cells at the concentration of 30 mu M.

Automated summary

Amestolkins A and B, two previously undescribed phthalides, were isolated from Talaromyces amestolkiae. They exhibited anti-neuroinflammatory activity by inhibiting the gene expressions of proinflammatory factors and attenuating the excretion of inducible nitric oxide synthase in BV-2 microglial cells.