Oviposition responses of gravid Aedes aegypti Linn. Mosquitoes (Diptera: Culicidae) to natural organic infusions under laboratory condition

The aim of this study was to investigate the effectiveness of different concentrations of three organic infusions as an oviposition attractancy agents of gravid Ae. aegypti mosquito under laboratory condition. Three organic infusions namely, Costus igneus Nak (Insulin plant leaf), Psidium guajava Linn (Guava tree leaf), and Cicer arietinum Linn (Black chickpea) were prepared by fermentation process and bioassays such as choice bioassay and comparison bioassay were performed to assess its oviposition attractiveness. In the choice bioassay, four different concentrations namely, 5%, 10%, 15%, 50% of each infusion was tested with gravid female Ae. aegypti and the oviposition results were compared to the water control. The results showed that the oviposition attractiveness was significantly differed among the different concentrations of three infusions. The highest mean oviposition was observed to be 457.3 +/- 22.9 in 15% insulin plant leave infusion and it was 192.6 +/- 11.5 in water control (F = 49.64; P = <0.001; OAI = +0.40). The best resulted concentration of the infusions in the choice bioassay was further studied and compared to the positive control (10% hay infusion). The highest mean oviposition was recorded in comparison bioassay was 475.3 +/- 10.7 in 15% insulin plant leaf infusion and it was 235.3 +/- 9.9 in 10% hay infusion (F = 18.71; P = <0.001; OAI = +0.38). These findings suggest that the insulin plant leaf infusion could be used in ovitraps as an effective organic oviposition attractant in Ae. aegypti mosquito surveillance and control program.

Automated summary

The study found that there were significant differences in oviposition attractiveness among the different concentrations of three organic infusions in gravid Ae. aegypti mosquitoes, with the insulin plant leaf infusion showing the highest oviposition attractiveness at 15% concentration. These findings suggest that the insulin plant leaf infusion could be used as an effective organic oviposition attractant in surveillance and control programs for Ae. aegypti mosquitoes.