期刊
JOURNAL OF APPLIED MICROBIOLOGY
卷 133, 期 3, 页码 1308-1321出版社
OXFORD UNIV PRESS
DOI: 10.1111/jam.15537
关键词
anti-fungal drug resistance; Candida-cidal activity; exo-1; 3 beta glucan synthase; phytoactive compounds; snow mountain garlic
资金
- Defence Institute of High Altitude Research, Defence Research and Development Organization, Ministry of Defence, Government of India
This study aimed to compare the volatile phytocomposition of snow mountain garlic (SMG) with normal garlic and investigate its anti-Candida efficacy against multi-drug resistant isolates of Candida species. The results showed that SMG has superior fungicidal power against C. albicans and C. glabrata compared to normal garlic. GC-MS profiling revealed unique volatile compounds and higher saponins content in SMG. In-silico analysis identified a potential inhibitor in SMG, suggesting a plausible anti-Candida mechanism. This study provides valuable insights into the potential use of SMG as a fungal phytotherapy agent.
Aim The study aimed to profile the volatile phytocomposition of snow mountain garlic (SMG) compared to normal garlic and investigate the anti-Candida efficacy against clinically relevant multi-drug resistant isolates of Candida species. Methods and Results Herein, SMG has shown significantly superior fungicidal power at 2x-MIC dose against C. albicans and C. glabrata in killing kinetic evaluation unlike the fungistatic effect of normal garlic. GC-MS headspace-based profiling of SMG showed 5 unique volatile compounds and a 5-fold higher content of saponins than normal garlic. In an in-silico analysis, cholesta-4,6-dien-3-ol,(3-beta) was uniquely identified in SMG as a potential inhibitor with high binding affinity to the active site of exo-1,3-betaglucan synthase, an established anti-candida drug target crucial for the biofilm matrix formation, thus suggesting a plausible anti-Candida mechanism. Conclusion The in-vitro and in-silico studies have demonstrated the Candida-cidal and anti-biofilm activities of SMG, distinguishing it from the Candida-static efficacy of normal garlic. Significance and Impact of the study This is the first report that identifies several phytochemical signatures of SMG along with a potential anti-Candida compound, that is cholesta-4,6-dien-3-ol,(3-beta)-, which appears worthy of detailed studies in the future to explore the utility of SMG as a fungal phytotherapy agent, especially against drug-resistant Candida sp.
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