期刊
JOURNAL OF AOAC INTERNATIONAL
卷 105, 期 4, 页码 941-949出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/jaoacint/qsac038
关键词
-
资金
- Natural Science Foundation of Hebei Province, China [C2015321005]
The study developed a fast analysis method to detect residues of vancomycin and norvancomycin in milk, with the limit of detection and quantitation being 0.15 and 0.5 μg/kg, respectively. The correlation coefficients for vancomycin and norvancomycin concentration ranged from 0 to 200 ng/mL were greater than 0.9983, indicating the method's suitability for rapid analysis of these residues in milk.
Background Vancomycin and norvancomycin, as potent antibacterial retention drugs, were used illegally on animals bred for food, which directly affected the quality and safety of animal-derived food, and even harmed human health. Objective A fast analysis method, which was adopted to detect residues of vancomycin and norvancomycin in milk, was implemented on a chromatographic system containing online solid-phase extraction (SPE) device that combined with high-resolution mass spectrometer (HRMS). Method First, the analytes were added to the blank milk sample were extracted with water [containing 0.1% trifluoroacetic acid (TFA)]-acetonitrile (ACN) (8:2, v/v), and then were purified and enriched on a C-18-XL column, whereafter eluted from the purification column onto the analytical column (Shiseido Capcell Pak ADME column) for chromatographic separation prior to hybrid quadrupole-Orbitrap (Q-Orbitrap) detection. Results The results showed that the limit of detection (LOD) for each analyte and the limit of quantitation (LOQ) were 0.15 and 0.5 mu g/kg, respectively. The correlation coefficient(s) of vancomycin and norvancomycin ranged from 0 to 200 ng/mL were greater than 0.9983. Conclusions These validations reflected that it was suitable for the established method to rapidly analyze vancomycin and norvancomycin residues in milk.
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