期刊
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
卷 150, 期 4, 页码 965-+出版社
MOSBY-ELSEVIER
DOI: 10.1016/j.jaci.2022.02.026
关键词
Lipids; asthma; nasal airway epithelium; proresolving lipids; oxylipins; resolvins; PUFAs; EPA; DHA; AA
资金
- National Institutes of Health (NIH)-National Heart, Lung, and Blood Institute (NHLBI) [R01HL104608]
- NIH-National Center for Research Resources [1S10OD010366-01A1]
- NIH-NHLBI [R01HL123385]
- Programa de Apoio a Nucleos Emergentes (PRONEM)-Fundacao de Amparoa Pesquisa do Estado da Bahia (FAPESB) [009/2014, PNE0003/2014, 8305/2014]
By analyzing nasal epithelial samples, we found that lipid mediators are detectable and can distinguish asthma cases from control subjects, suggesting their potential involvement in the pathogenesis of asthma.
Background: Lipid mediators, bioactive products of polyunsaturated fatty acid metabolism, contribute to inflammation initiation and resolution in allergic diseases; however, their presence in lung-related biosamples has not been fully described. Objective: We aimed to quantify lipid mediators in the nasal airway epithelium and characterize preliminary associations with asthma. Methods: Using liquid chromatography-mass spectrometry, we conducted a pilot study to quantify 56 lipid mediators from nasal epithelial samples collected from 11 female participants of an outpatient asthma clinic and community controls (aged 30-55 years). We examined the presence of each compound using descriptive statistics to test whether lipid mediators could distinguish subjects with asthma (n = 8) from control subjects (n = 3) using linear regression and partial least squares discriminant analysis. Results: Fifteen lipid mediators were detectable in all samples, including resolvin (Rv) D5 (RvDS), with the highest median concentrations (in pg/mu g protein) of 13-HODE (126.481), 15-HETE (32.869), and 13-OxoODE (13.251). From linear regression adjusted for age, prostaglandin E-2 (PGE2) had a trend (P < .1) for higher concentrations in patients with severe asthma compared to controls (mean difference, 0.95; 95% confidence interval, -0.04 to 1.95). Asthma patients had higher scores on principal component 3 compared to controls (mean difference, 2.42; 95% confidence interval, 0.89 to 3.96), which represented lower levels of proresolving 15-HEPE, 19,20-DiHDPA, RvDS, 14-HDHA, 17-HDHA, and 13-HOTrE. Most of these compounds were best at discriminating asthma cases from controls in partial least squares discriminant analysis. Conclusion: Lipid mediators are detectable in the nasal epithelium, and their levels distinguish asthma cases from controls.
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