Butyl Group Distribution, Intestinal Digestion, and Colonic Fermentation Characteristics of Different Butyrylated Starches

Despite being a promising butyrate carrier, butyrylated starch remains poorly understood in terms of the correlation between starch structure and fermentation characteristics. Herein, three butyrylated starches derived from different botanical sources were prepared with a similar degree of substitution. Raman microscopy and water contact angle analysis suggested that a relatively large proportion of butyl group substitutions occurred within the interior of butyrylated waxy maize starch (B-WMS) granules. In vitro digestion results showed that branch points provided butyl groups with a specific protection from enzymatic hydrolysis, whereas butyl groups significantly increased the resistant starch content of butyrylated starch. Moreover, the porous morphology with less distributed butyl groups on the granular surface contributed to a faster fermentation rate in B-WMS. The current study reveals the influence of botanical origin on butyl group distribution, which in turn plays a pivotal role in regulating the intestinal digestion and colonic fermentation of butyrylated starch.

Automated summary

This study investigates the influence of botanical origin on the distribution of butyl groups in butyrylated starch and their role in regulating digestion and fermentation. The findings suggest that the distribution of butyl groups within the starch granules and the presence of branch points can affect enzymatic hydrolysis and resistant starch content. The porous morphology of the starch granules also contributes to faster fermentation.