Effects of Molecular Weights -Assisted Enzymatic Hydrolysis on Antioxidant and Anticancer Activities of Liza abu Muscle Protein Hydrolysates

In this study, enzymatic hydrolysis of Liza abu muscle protein was used by various commercial enzymes to produce bioactive peptides with antioxidant and anti-cancer properties. Hydrolysis protein was produced at 58 degrees C using alcalase and flavourzyme commercial enzymes and alcalase + flavourzyme (1:1) at pHs 7 and 8.5 in the ratio of 1% of the enzyme to prototype protein. The results showed that alcalase enzyme produced hydrolyzed protein with a degree of hydrolysis 36%, about 80.67% protein content, and 70.72% protein recovery (P < 0.05). In the next phase, the hydrolyzed proteins were divided into three sections with different molecular weights (> 3, 10, and 30 kDa). Based on our findings, the type of enzyme and molecular weight of the protein are effective in the production of bioactive peptides so that the highest antioxidant activity (DPPH free radical scavenging activity, ferric reducing power, Fe2+ chelating activity, and ABTS free radical scavenging activity) and anti-cancer properties (against colon cancer cells and Hela cancer cells) were mostly related to alcalase enzyme (P < 0.05) and in some cases, the alcalase + flavourzyme enzyme showed appropriate results. In all cases, hydrolyzed proteins with a molecular weight of less than 3 kDa showed higher antioxidant and anti-cancer properties (P < 0.05). Overall, it seems that the hydrolyzed protein of L. abu muscle has high antioxidant and anti-cancer activity, which can be used as protein supplements in foods and diets.

Automated summary

This study utilized enzymatic hydrolysis to produce bioactive peptides with antioxidant and anti-cancer properties from Liza abu muscle protein. Alcalase enzyme showed the highest antioxidant and anti-cancer activity, while hydrolyzed proteins with a molecular weight of less than 3 kDa exhibited the best results.