ArabidopsisKCS5 and KCS6 Play Redundant Roles in Wax Synthesis

3-ketoacyl-CoA synthases (KCSs), as components of a fatty acid elongase (FAE) complex, play key roles in determining the chain length of very-long-chain fatty acids (VLCFAs). KCS6, taking a predominate role during the elongation from C26 to C28, is well known to play an important role in wax synthesis. KCS5 is one paralog of KCS6 and its role in wax synthesis remains unknown. Wax phenotype analysis showed that in kcs5 mutants, the total amounts of wax components derived from carbon 32 (C32) and C34 were apparently decreased in leaves, and those of C26 to C32 derivatives were obviously decreased in flowers. Heterologous yeast expression analysis showed that KCS5 alone displayed specificity towards C24 to C28 acids, and its coordination with CER2 and CER26 catalyzed the elongation of acids exceeding C28, especially displaying higher activity towards C28 acids than KCS6. BiLC experiments identified that KCS5 physically interacts with CER2 and CER26. Wax phenotype analysis of different organs in kcs5 and kcs6 single or double mutants showed that KCS6 mutation causes greater effects on the wax synthesis than KCS5 mutation in the tested organs, and simultaneous repression of both protein activities caused additive effects, suggesting that during the wax biosynthesis process, KCS5 and KCS6 play redundant roles, among which KCS6 plays a major role. In addition, simultaneous mutations of two genes nearly block drought-induced wax production, indicating that the reactions catalyzed by KCS5 and KCS6 play a critical role in the wax biosynthesis in response to drought.

Automated summary

3-ketoacyl-CoA synthases (KCSs) are key components of a fatty acid elongase (FAE) complex that determines the chain length of very-long-chain fatty acids (VLCFAs). KCS6 is well known for its role in wax synthesis, while the role of KCS5 remains unknown. This study showed that KCS5 interacts with CER2 and CER26 and displays specificity towards C24 to C28 acids. The results also suggest that KCS5 and KCS6 play redundant roles in wax biosynthesis, with KCS6 playing a major role. The mutations of both KCS5 and KCS6 nearly blocked drought-induced wax production.