期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 64, 期 37, 页码 6939-6949出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.6b02314
关键词
alpha-glucosidase; apigenin; inhibition mechanism; synergy; isobologram
资金
- National Natural Science Foundation of China [31460422, 31060210]
- Natural Science Foundation of Jiangxi Province [20143ACB20006]
- Foundation of Jiangxi Provincial Office of Education [GJJ150187]
- Objective-Oriented Project of State Key Laboratory of Food Science and Technology [SKLF-ZZA-201612]
Inhibition of alpha-glucosidase activity may suppress postprandial hyperglycemia. The inhibition kinetic analysis showed that apigenin reversibly inhibited alpha-glucosidase activity with an IC50 value of (10.5 +/- 0.05) x 10(-6) mol L-1, and the inhibition was in a noncompetitive manner through a monophasic kinetic process. The fluorescence quenching and conformational changes determined by fluorescence and circular dichroism were due to the formation of an alpha-glucosidase apigenin complex, and the binding was mainly driven by hydrophobic interactions and hydrogen bonding. The molecular simulation showed that apigenin bound to a site close to the active site of alpha-glucosidase, which may induce the channel closure to prevent the access of substrate, eventually leading to the inhibition of alpha-glueo idase. Isobolographic analysis of the interaction between myricetin and apigenin or morin showed that both of them exhibited synergistic effects at low concentrations and tended to exhibit additive or antagonistic interaction at high concentrations.
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