期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 64, 期 9, 页码 1921-1931出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b05610
关键词
polysaccharide; Lepidium meyenii; structural characterization; immunomodulatory activity
资金
- Open Project Program of Provincial Key Laboratory of Green Processing Technology and Product Safety of Natural Products [201304]
- National Natural Science Foundation of China [31201330]
A novel polysaccharide named as MC-1 was isolated from the roots of Lepidium meyenii using a water extraction method. Structural characterization revealed that MC-1 had an average molecular weight of 11.3 kDa and consisted of arabinose (26.21%), mannose (11.81%), glucose (53.66%), and galactose (8.32%). The main linkage types of MC-1 were proven to be (1 -> 5)-alpha-L-Ara, (1 -> 3)-alpha-L-Man, (1 -> 2,6)-alpha-L-Man, (1 -> )-alpha-D-Glc, (1 -> 4)-alpha-n-Glc, (1 -> 6)-alpha-D-Glc and (1 -> 6)-alpha-D-Gal by methylation analysis, periodate oxidation-Smith degradation and NMR analysis. The immunostimulating assay indicated that MC-1 could significantly enhance the pinocytic and phagocytic capacity and promote the NO, TNF-alpha, and IL-6 secretion of RAW 264.7 cells, involving toll-like receptor 2, complement receptor 3, and mannose receptor mainly. These results suggested the potential utilization of MC-1 as an attractive functional food supplement candidate for hypoimmunity population.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据